| | |  | | | | Record from Web of Science® | | | | | | |
| INITIAL ASSESSMENT OF HUMAN GENE DIVERSITY AND EXPRESSION PATTERNS BASED UPON 83-MILLION NUCLEOTIDES OF CDNA SEQUENCE |
|
|
| Author(s): ADAMS MD, KERLAVAGE AR, FLEISCHMANN RD, FULDNER RA, BULT CJ, LEE NH, KIRKNESS EF, WEINSTOCK KG, GOCAYNE JD, WHITE O, SUTTON G, BLAKE JA, BRANDON RG, CHIU MW, CLAYTON RA, CLINE RT, COTTON MD, EARLEHUGHES J, FINE LD, FITZGERALD LM, FITZHUGH WM, FRITCHMAN JL, GEOGHAGEN NSM, GLODEK A, GNEHM CL, HANNA MC, HEDBLOM E, HINKLE PS, KELLEY JM, KLIMEK KM, KELLEY JC, LIU LI, MARMAROS SM, MERRICK JM, MORENOPALANQUES RF, MCDONALD LA, NGUYEN DT, PELLEGRINO SM, PHILLIPS CA, RYDER SE, SCOTT JL, SAUDEK DM, SHIRLEY R, SMALL KV, SPRIGGS TA, UTTERBACK TR, WELDMAN JF, LI Y, BARTHLOW R, BEDNARIK DP, CAO LA, CEPEDA MA, COLEMAN TA, COLLINS EJ, DIMKE D, FENG P, FERRIE A, FISCHER C, HASTINGS GA, HE WW, HU JS, HUDDLESTON KA, GREENE JM, GRUBER J, HUDSON P, KIM A, KOZAK DL, KUNSCH C, JI HJ, LI HD, MEISSNER PS, OLSEN H, RAYMOND L, WEI YF, WING J, XU C, YU GL, RUBEN SM, DILLON PJ, FANNON MR, ROSEN CA, HASELTINE WA, FIELDS C, FRASER CM, VENTER JC |
| Source: NATURE Volume: 377 Pages: 3-& Supplement: Suppl. S Published: SEP 28 1995 |
| Times Cited: 639 References: 69 |
| Abstract: In an effort to identify new genes and analyse their expression patterns, 174,472 partial complementary DNA sequences (expressed sequence tags (ESTs)), totalling more than 52 million nucleotides of human DNA sequence, have been generated from 300 cDNA libraries constructed from 37 distinct organs and tissues. These ESTs have been combined with an additional 118,406 ESTs from the database dbEST, for a total of 83 million nucleotides, and treated as a shotgun sequence assembly project. The assembly process yielded 29,599 distinct tentative human consensus (THC) sequences and 58,384 non-overlapping ESTs. Of these 87,983 distinct sequences, 10,214 further characterize previously known genes based on statistically significant similarity to sequences in the available databases; the remainder identify previously unknown genes. Thirty tissues were sampled by over 1,000 ESTs each; only eight genes were matched by ESTs from all 30 tissues, and 227 genes were represented in 20 or more of the tissues sampled with more than 1,000 ESTs. Approximately 40% of identified human genes appear to be associated with basic energy metabolism, cell structure, homeostasis and cell division, 22% with RNA and protein synthesis and processing, and 12% with cell signalling and communication. |
| Document Type: Article |
| Language: English |
Addresses:
1. INST GENOM RES, ROCKVILLE, MD 20850 USA
2. HUMAN GENOME SCI INC, ROCKVILLE, MD 20850 USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: TA343 |
| ISSN: 0028-0836 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |