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| CELL-LINES FOR THE PRODUCTION OF RECOMBINANT ADENOASSOCIATED VIRUS |
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| Author(s): CLARK KR, VOULGAROPOULOU F, FRALEY DM, JOHNSON PR |
| Source: HUMAN GENE THERAPY Volume: 6 Issue: 10 Pages: 1329-1341 Published: OCT 1995 |
| Times Cited: 140 References: 56 |
| Abstract: Adeno-associated virus (AAV) is a replication-defective parvovirus that is being developed as a vector for human gene transfer, However, a major obstacle to commonplace usage of AAV vectors is the production of recombinant virions (rAAV) in sufficient quantities for not only human trials, but also for preclinical studies of basic biology, toxicology, and efficacy, Unfortunately, current methods for large-scale production are cumbersome and expensive, We have developed a simplified method for generating rAAV by establishing neomycin-resistant cell lines containing copies of the AAV rep-cap genes and a rAAV vector, After infection with adenovirus, these cell lines were shown to produce infectious rAAV in relatively high titer, This method eliminates the need for exogenous DNA transfection and scale-up procedures are limited only by the normal constraints of growing cells in culture. |
| Document Type: Article |
| Language: English |
Addresses:
1. OHIO STATE UNIV, CHILDRENS HOSP, CHILDRENS HOSP RES FDN, COLUMBUS, OH 43205 USA
2. OHIO STATE UNIV, DEPT PEDIAT, COLUMBUS, OH 43205 USA
3. OHIO STATE UNIV, COLL MED, DEPT MED MICROBIOL & IMMUNOL, COLUMBUS, OH 43205 USA
4. OHIO STATE UNIV, PROGRAM MOLEC & CELLULAR DEV BIOL, COLUMBUS, OH 43205 USA |
| Publisher: MARY ANN LIEBERT INC PUBL, 2 MADISON AVENUE, LARCHMONT, NY 10538 |
| Subject Category: Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental |
| IDS Number: TA720 |
| ISSN: 1043-0342 |
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