| INTRINSIC GLYCOSYLATION POTENTIALS OF INSECT-CELL CULTURES AND INSECT LARVAE |
|
|
| Author(s): DAVIS TR, WOOD HA |
| Source: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL Volume: 31 Issue: 9 Pages: 659-663 Published: OCT 1995 |
| Times Cited: 48 References: 31 |
| Abstract: The glycosylation and subsequent processing of native and recombinant glycoproteins expressed in established insect cell lines and insect larvae were compared. The Spodoptera frugiperda (Sf21) and Trichoplusia ni (TN-368 and BTI-Tn-5B1-4) cell lines possessed several intrinsic glycoproteins that are modified with both N- and O-linked oligosaccharides. The N-linked oligosaccharides were identified as both the simple (high mannose) and complex (containing sialic acid) types. Similarly, the T. ni larvae also possessed intrinsic glycoproteins that were modified with O-Linked and simple and complex N-linked oligosaccharides. Additionally, human placental, secreted alkaline phosphatase (SEAP) produced during replication of a recombinant baculovirus in T. ni larvae was modified with complex oligosaccharide having sialic acid linked alpha(2-6) to galactose. |
| Document Type: Article |
| Language: English |
Addresses:
1. CORNELL UNIV, BOYCE THOMPSON INST PLANT RES, ITHACA, NY 14853 USA |
| Publisher: SOC IN VITRO BIOLOGY, 8815 CENTRE PARK DRIVE SUITE 210, COLUMBIA, MD 21045 |
| Subject Category: Cell Biology; Developmental Biology |
| IDS Number: TB080 |
| ISSN: 1071-2690 |