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| SOMATIC HYPERMUTATION IN LOW-GRADE MUCOSA-ASSOCIATED LYMPHOID TISSUE-TYPE B-CELL LYMPHOMA |
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| Author(s): QIN YF, GREINER A, TRUNK MJF, SCHMAUSSER B, OTT MM, MULLERHERMELINK HK |
| Source: BLOOD Volume: 86 Issue: 9 Pages: 3528-3534 Published: NOV 1 1995 |
| Times Cited: 127 References: 53 |
| Abstract: The origin of low-grade mucosa-associated lymphoid tissue (MALT)-type B-cell lymphoma is still unclear, Using a novel two-step procedure, we have sequenced the Ig VH genes expressed by cells from four patients with gastric low-grade MALT-type lymphoma. The nucleotide sequences of the complementarity determining region 3 (CDR3) of the genomic DNA were first amplified using consensus oligonucleotide primers, then sequenced, Based on the CDR3 sequence amplified from each MALT lymphoma, individual tumor-specific primers were synthesized and used directly in the polymerase chain reaction (PCR) to analyze the sequences of their Ig heavy-chain variable region. When compared with the germ-line sequence, many nucleotide substitutions, mainly in the CDRs, were found in the variable gene sequences of the four MALT lymphomas, The mutations showed a high replacement-to-silent ratio and were distributed in a way which suggested that the tumor cells had been positively selected through their antigen receptor, Our findings indicate that the MALT-type lymphoma B cells are hypermutated postgerminal center lymphocytes that have undergone antigen selection. (C) 1995 by The American Society of Hematology. |
| Document Type: Article |
| Language: English |
| Reprint Address: QIN, YF (reprint author), UNIV WURZBURG, INST PATHOL, JOSEF SCHNEIDER STR 2, D-97080 WURZBURG, GERMANY |
| Publisher: W B SAUNDERS CO, INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 |
| Subject Category: Hematology |
| IDS Number: TB163 |
| ISSN: 0006-4971 |
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