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| EARLY REDISTRIBUTION OF PLASMA-MEMBRANE PHOSPHATIDYLSERINE IS A GENERAL FEATURE OF APOPTOSIS REGARDLESS OF THE INITIATING STIMULUS - INHIBITION BY OVEREXPRESSION OF BCL-2 AND ABL |
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| Author(s): MARTIN SJ, REUTELINGSPERGER CPM, MCGAHON AJ, RADER JA, VANSCHIE RCAA, LAFACE DM, GREEN DR |
| Source: JOURNAL OF EXPERIMENTAL MEDICINE Volume: 182 Issue: 5 Pages: 1545-1556 Published: NOV 1 1995 |
| Times Cited: 1,614 References: 51 |
| Abstract: A critical event during programmed cell death (PCD) appears to be the acquisition of plasma membrane (PM) changes that allows phagocytes to recognize and engulf these cells before they rupture. The majority of PCD seen in higher organisms exhibits strikingly similar morphological features, and this form of PCD has been termed apoptosis. The nature of the PM changes that occur on apoptotic cells remains poorly defined. In this study, we have used a phosphatidylserine (PS)-binding protein (annexin V) as a specific probe to detect redistribution of this phospholipid, which is normally confined to the inner PM leaflet, during apoptosis. Here we show that PS externalization is an early and widespread event during apoptosis of a variety of murine and human cell types, regardless of the initiating stimulus, and precedes several other events normally associated with this mode of cell death. We also report that, under conditions in which the morphological features of apoptosis were prevented (macromolecular synthesis inhibition, overexpression of Bcl-2 or Abl), the appearance of PS on the external leaflet of the PM was similarly prevented. These data are compatible with the notion that activation of an inside-outside PS translocase is an early and widespread event during apoptosis. |
| Document Type: Article |
| Language: English |
| Reprint Address: MARTIN, SJ (reprint author), LA JOLLA INST ALLERGY & IMMUNOL, DIV CELLULAR IMMUNOL, 11149 N TORREY PINES RD, LA JOLLA, CA 92037 USA |
Addresses:
1. UNIV LIMBURG, DEPT BIOCHEM, 6200 MD MAASTRICHT, NETHERLANDS
2. UCL, SCH MED, DEPT IMMUNOL, LONDON W1N 8AA, ENGLAND |
| Publisher: ROCKEFELLER UNIV PRESS, 222 E 70TH STREET, NEW YORK, NY 10021 |
| Subject Category: Immunology; Medicine, Research & Experimental |
| IDS Number: TB894 |
| ISSN: 0022-1007 |
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