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| LOW SPECIFICITY OF CYTOKERATIN 19 REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION ANALYSES FOR DETECTION OF HEMATOGENOUS LUNG-CANCER DISSEMINATION |
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| Author(s): KRISMANN M, TODT B, SCHRODER J, GAREIS D, MULLER KM, SEEBER S, SCHUTTE J |
| Source: JOURNAL OF CLINICAL ONCOLOGY Volume: 13 Issue: 11 Pages: 2769-2775 Published: NOV 1995 |
| Times Cited: 192 References: 36 |
| Abstract: Purpose: Sensitive detection of systemic tumor dissemination in lung cancer patients is important for selection of appropriate treatment modalities. Based on recent promising data that showed reverse transcriptase-polymerase chain reaction (RT-PCR) analyses for cytokeratin 19 (CK-19) expression in peripheral-blood or bone marrow samples to be a rapid and highly sensitive method for detection of hematogenous tumor dissemination in patients with breast and prostate cancer, we evaluated the specificity of this assay system in lung cancer patients and a large number of healthy controls. Patients and Methods: We examined CK-19 mRNA expression by RT-PCR in 17 lung cancer cell lines and in peripheral-blood samples of 50 lung tumor patients and 65 healthy controls.
Results: Expression of CK-19 mRNA was observed in ail lung cancer cell lines and in 50% of peripheral-blood samples from lung tumor patients. However, under the experimental conditions analyzed, at least 20% of the control samples were positive for CK-19 mRNA expression.
Conclusion: Contrary to prior reports, RT-PCR may detect non-tissue-specific constitutive low-level (illegitimate) expression of CK-19 mRNA in peripheral-blood mononuclear (PBMN) cells in a significant number of healthy controls. This finding may not only hamper the use of this assay system in lung cancer patients, but also questions its proposed applicability in patients with other epithelial tumors such as breast and prostate cancer.
(C) 1995 by American Society of Clinical Oncology.
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| Document Type: Article |
| Language: English |
Addresses:
1. UNIV ESSEN GESAMTHSCH KLINIKUM, W DEUTSCHES TUMORZENTRUM ESSEN, INNERE KLIN & POLIKLIN TUMORFORSCH, D-45122 ESSEN, GERMANY
2. BERUFSGENOSSENSCH KLINIKEN BERGMANNSHEIL, INST PATHOL, BOCHUM, GERMANY |
| Publisher: W B SAUNDERS CO, INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 |
| Subject Category: Oncology |
| IDS Number: TC986 |
| ISSN: 0732-183X |
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