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| ALLELIC POLYMORPHISM IN IL-1-BETA AND IL-1 RECEPTOR ANTAGONIST (IL-1RA) GENES IN INFLAMMATORY BOWEL-DISEASE |
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| Author(s): BIOQUE G, CRUSIUS JBA, KOUTROUBAKIS I, BOUMA G, KOSTENSE PJ, MEUWISSEN SGM, PENA AS |
| Source: CLINICAL AND EXPERIMENTAL IMMUNOLOGY Volume: 102 Issue: 2 Pages: 379-383 Published: NOV 1995 |
| Times Cited: 153 References: 32 |
| Abstract: Recent reports have shown that allele 2 of the IL-1 receptor antagonist (IL-1Ra) gene is overrepresented in ulcerative colitis (UC). Healthy individuals carrying allele 2 of this gene have increased production of IL-1Ra protein. Since the final outcome of the biological effects of IL-1 beta may depend on the relative proportion of these two cytokines, we have studied if a TaqI polymorphism in the IL-1 beta gene, which is relevant to IL-1 beta protein production, may be involved in the genetic susceptibility to UC and Crohn's disease (CD), in association with the established IL-1Ra gene polymorphism. Polymorphisms in the closely linked genes for IL-1 beta and IL-1Ra were typed in 100 unrelated Dutch patients with UC, 79 with CD, and 71 healthy controls. The polymorphic regions in exon 5 of the IL-1 beta gene and in intron 2 of the IL-1Ra gene, were studied by polymerase chain reaction (PCR)-based methods. The IL-1 beta allele frequencies in UC and CD patients did not differ from those in healthy controls. In order to study if the IL-1 beta gene polymorphism might participate synergistically with the IL-1Ra gene polymorphism in susceptibility to UC and CD, individuals were distributed into carriers and non-carriers of allele 2 of the genes encoding IL-1 beta and IL-1Ra, in each of the patient groups and controls. Results indicated a significant association of this pair of genes, estimated by the odds ratio (OR) after performing Fisher's exact test, in the UC group (P = 0.023, OR = 2.81), as well as in the CD group (P = 0.01, OR = 3.79). Thus, non-carriers of IL-1 beta allele 2 were more often present in the subgroup of patients carrying the IL-1Ra allele 2. By contrast, no association of these alleles was detected in the group of healthy controls (P = 1.00, OR = 0.92). These results suggest that the IL-1beta>/IL-1Ra allelic cluster may participate in defining the biological basis of predisposition to chronic inflammatory bowel diseases. |
| Document Type: Article |
| Language: English |
Addresses:
1. FREE UNIV AMSTERDAM HOSP, DEPT GASTROENTEROL, 1007 MB AMSTERDAM, NETHERLANDS
2. FREE UNIV AMSTERDAM HOSP, DEPT EPIDEMIOL & BIOSTAT, 1007 MB AMSTERDAM, NETHERLANDS |
| Publisher: BLACKWELL SCIENCE LTD, OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL |
| Subject Category: Immunology |
| IDS Number: TD210 |
| ISSN: 0009-9104 |
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