| SOLUTION STRUCTURE OF THE KLUYVEROMYCES-LACTIS LAC9 CD(2)CYS(6) DNA-BINDING DOMAIN |
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| Author(s): GARDNER KH, ANDERSON SF, COLEMAN JE |
| Source: NATURE STRUCTURAL BIOLOGY Volume: 2 Issue: 10 Pages: 898-905 Published: OCT 1995 |
| Times Cited: 21 References: 45 |
| Abstract: The Zn(2)Cys(6) DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator Using H-1 NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of Cd-113-H-1 NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd-2 binuclear cluster with a network of amide proton-cysteinyl S gamma hydrogen bonds stablizing the cluster. Comparison with other Zn(2)Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding. |
| Document Type: Article |
| Language: English |
Addresses:
1. YALE UNIV, DEPT MOLEC BIOPHYS & BIOCHEM, NEW HAVEN, CT 06520 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| IDS Number: TD293 |
| ISSN: 1072-8368 |