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| SUPPRESSOR OF HAIRLESS DIRECTLY ACTIVATES TRANSCRIPTION OF ENHANCER OF SPLIT COMPLEX GENES IN RESPONSE TO NOTCH RECEPTOR ACTIVITY |
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| Author(s): BAILEY AM, POSAKONY JW |
| Source: GENES & DEVELOPMENT Volume: 9 Issue: 21 Pages: 2609-2622 Published: NOV 1 1995 |
| Times Cited: 348 References: 60 |
| Abstract: We have investigated the functional relationships among three loci that are required far multiple alternative cell fate decisions during adult peripheral neurogenesis in Drosophila: Notch (N), which encodes a transmembrane receptor protein, Suppressor of Hairless [Su(H)], which encodes a DNA-binding transcription factor, and the Enhancer of split gene complex [E(spl)-C], which includes seven transcription units that encode basic helix-loop-helix (bHLH) repressor proteins. We describe several lines of evidence establishing that Su(H) directly activates transcription of E(spl)-C genes in response to N receptor activity. Expression of an activated form of the N receptor leads to elevated and ectopic E(sp1)-C transcript accumulation and promoter activity in imaginal discs. We show that the proximal upstream regions of three E(spl)-C genes contain multiple specific binding sites for Su(H). The integrity of these sites, as well as Su(H) gene activity, are required not only for normal levels of expression of E(sp1)-C genes in imaginal disc proneural clusters, but also for their transcriptional response to hyperactivity of the N receptor. Our results establish Su(H) as a direct regulatory link between N receptor activity and the expression of E(spl)-C genes, extending the known linear structure of the N cell-cell signaling pathway. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV CALIF SAN DIEGO, DEPT BIOL, LA JOLLA, CA 92093 USA
2. UNIV CALIF SAN DIEGO, CTR MOLEC GENET, LA JOLLA, CA 92093 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 |
| Subject Category: Cell Biology; Developmental Biology; Genetics & Heredity |
| IDS Number: TD885 |
| ISSN: 0890-9369 |
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