| | |  | | | | Record from Web of Science® | | | | | | |
| UROCORTIN, A MAMMALIAN NEUROPEPTIDE RELATED TO FISH UROTENSIN-I AND TO CORTICOTROPIN-RELEASING FACTOR |
|
|
| Author(s): VAUGHAN J, DONALDSON C, BITTENCOURT J, PERRIN MH, LEWIS K, SUTTON S, CHAN R, TURNBULL AV, LOVEJOY D, RIVIER C, RIVIER J, SAWCHENKO PE, VALE W |
| Source: NATURE Volume: 378 Issue: 6554 Pages: 287-292 Published: NOV 16 1995 |
| Times Cited: 904 References: 30 |
| Abstract: Corticotropin-releasing factor (CRF), a peptide first isolated from mammalian brain(1), is critical in the regulation of the pituitary-adrenal axis, and in complementary stress-related endocrine, autonomic and behavioural responses(2). Fish urotensin I and amphibian sauvagine were considered to be homologues(3) of CRF until peptides even more closely related to CRF were identified in these same vertebrate classes(4,5). We have characterized another mammalian member of the CRF family and have localized its urotensin-like immunoreactivity to, and cloned related complementary DNAs from, a discrete rat midbrain region. The deduced protein encodes a peptide that we name urocortin, which is related to urotensin (63% sequence identity) and CRF (45% sequence identity). Synthetic urocortin evokes secretion of adrenocorticotropic hormone (ACTH) both in vitro and in vivo and binds and activates transfected type-1 CRF receptors(6,9), the subtype expressed by pituitary corticotropes. The coincidence of urotensin-like immunoreactivity with type-2 CRF receptors(10-13) in brain, and our observation that urocortin is more potent than CRF at binding and activating type-2 CRF receptors, as well as at inducing c-Fos (an index of cellular activation) in regions enriched in type-2 CRF receptors, indicate that this new peptide could be an endogenous ligand for type-2 CRF receptors. |
| Document Type: Article |
| Language: English |
Addresses:
1. SALK INST BIOL STUDIES, CLAYTON FDN LABS PEPTIDE BIOL, LA JOLLA, CA 92037 USA
2. SALK INST BIOL STUDIES, NEURONAL STRUCT & FUNCT LAB, LA JOLLA, CA 92037 USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: TE858 |
| ISSN: 0028-0836 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |