| | |  | | | | Record from Web of Science® | | | | | | |
| QUANTITATION AND PROGNOSTIC VALUE OF BREAST-CANCER ANGIOGENESIS - COMPARISON OF MICROVESSEL DENSITY, CHALKLEY COUNT, AND COMPUTER IMAGE-ANALYSIS |
|
|
| Author(s): FOX SB, LEEK RD, WEEKES MP, WHITEHOUSE RM, GATTER KC, HARRIS AL |
| Source: JOURNAL OF PATHOLOGY Volume: 177 Issue: 3 Pages: 275-283 Published: NOV 1995 |
| Times Cited: 280 References: 47 |
| Abstract: In some studies of breast cancer, quantitation of immunohistochemically highlighted microvessel 'hot spots' has been shown to be a powerful prognostic tool. However, the antibody used, the number and size of the 'hot spots' assessed, and the stratification of patients into high and low vascular groups vary between studies. Furthermore, little is known about the relationship between microvessel density and other vascular parameters. These uncertainties and the laborious nature of the technique make it unsuitable for diagnostic practice. Both manual and computerized image analysis techniques were used in this study to examine the relationship between microvessel density and the vascular parameters in different sized microscopic fields in a pilot series of 30 invasive breast carcinomas. Automated pixel analysis of immunohistochemical staining, Chalkley point counting, and observer subjective vascular grading were also assessed as more rapid methods of measuring tumour vascularity. A Chalkley count was also performed on a further 211 invasive breast carcinomas. Significant correlations were observed between manual microvessel density and luminal perimeter (r=0.6, P=0.0004), luminal area (r=0.56, P=0.002), and microvessel number (r=0.57, P=0.0009) by computerized analysis. There were also significant correlations between the microscopic hot spots of 0.155 mm(2) and 0.848 mm(2) for microvessel number (r=0.81, P<0.00005), luminal perimeter (r=0.78, P<0.00005), and luminal area (r=0.65, P=0.0001). In addition, a significant correlation was observed between microvessel density and both subjective vascular grade (P=0.002) and Chalkley count (P=0.0001). A significant reduction in overall survival was observed between patients stratified by Chalkley count in both a univariate (P=0.02) and a multivariate (P=0.05) analysis in the 211 invasive breast carcinomas. These findings show that Chalkley counting is a rapid method of quantifying tumour angiogenesis and gives independent prognostic information which might be useful in diagnostic practice. |
| Document Type: Article |
| Language: English |
| Reprint Address: FOX, SB (reprint author), UNIV OXFORD, JOHN RADCLIFFE HOSP, DEPT CELLULAR SCI, LEVEL 4, ACAD BLOCK, OXFORD OX3 9DU, ENGLAND |
Addresses:
1. JOHN RADCLIFFE HOSP, INST MOLEC MED, IMPERIAL CANC RES FUND, MOLEC ONCOL LAB, OXFORD OX3 9DU, ENGLAND |
| Publisher: JOHN WILEY & SONS LTD, BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD |
| Subject Category: Oncology; Pathology |
| IDS Number: TF184 |
| ISSN: 0022-3417 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |