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| DETECTION OF SECTION-SPECIFIC RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS IN LILIUM |
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| Author(s): YAMAGISHI M |
| Source: THEORETICAL AND APPLIED GENETICS Volume: 91 Issue: 6-7 Pages: 830-835 Published: NOV 1995 |
| Times Cited: 35 References: 20 |
| Abstract: Random amplified polymorphic DNA (RAPD) markers were utilized for the identification of Lilium species and inter-specific hybrids, The optimum annealing temperature of the polymerase chain reaction (PCR) for the RAPD assay in Lilium was 54 degrees C, which is relatively higher than the temperature used for other genera reported by previous researchers. Among 76 primers used to amplify genomic DNA by PCR, 18 primers (24%) generated polymorphic DNA fragments in Lilium species and hybrids. Cultivars were also identified by RAPD markers. Some amplified fragments were unique to species of each section and to hybrids derived from these species; that is, they were the section-specific DNA markers, Sections, Sinomartagon, Leucolirion b, Leucolirion a and Archelirion could be identified by 6 section-specific markers amplified with five primers, Seven inter-section hybrids showed the section-specific bands of both parental sections, indicating that these markers would be useful for identifying the parental sections of inter-section hybrids. |
| Document Type: Article |
| Language: English |
| Reprint Address: YAMAGISHI, M (reprint author), ISHIKAWA AGR COLL, AGR RESOURCES RES INST, NONOICHI, ISHIKAWA 921 JAPAN |
| Publisher: SPRINGER VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 |
| Subject Category: Agronomy; Plant Sciences; Genetics & Heredity; Horticulture |
| IDS Number: TH319 |
| ISSN: 0040-5752 |
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