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| GENE TARGETING IN MOUSE EMBRYONIC STEM-CELLS WITH AN ADENOVIRAL VECTOR |
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| Author(s): MITANI K, WAKAMIYA M, HASTY P, GRAHAM FL, BRADLEY A, CASKEY CT |
| Source: SOMATIC CELL AND MOLECULAR GENETICS Volume: 21 Issue: 4 Pages: 221-231 Published: JUL 1995 |
| Times Cited: 21 References: 28 |
| Abstract: We examined the ability of an EI, E3-defective adenoviral vector to act as a substrate for homologous recombination with chromosomal DNA by including host chromosomal sequence from the mouse Fgr locus that also contained a selectable marker. After infection of mouse embryonic stem cells, stable integration was selected for neomycin resistance and the efficiency of homologous recombination was evaluated. The adenoviral vector was capable of infecting mouse embryonic stem cells efficiently. Between 30-50% of the input virus reached the nuclei after 24 hours of infection. Surprisingly, even without negative selection, 25-40% of the integration resulted from homologous recombination at m.o.i 10 and 100, although the absolute efficiency of integration was low. Our results suggest that it is possible to modify the structure of an adenoviral vector to achieve a high gene targeting efficiency, resulting in regulated and long-term expression of an introduced gene. |
| Document Type: Article |
| Language: English |
Addresses:
1. BAYLOR COLL MED, DEPT MOLEC & HUMAN GENET, HOUSTON, TX 77030 USA
2. BAYLOR COLL MED, HOWARD HUGHES MED INST, HOUSTON, TX 77030 USA
3. UNIV TEXAS, MD ANDERSON CANC CTR, DEPT BIOCHEM & MOLEC BIOL, HOUSTON, TX 77030 USA
4. MCMASTER UNIV, DEPT BIOL, TORONTO, ON L8S 4K1 CANADA |
| Publisher: PLENUM PUBL CORP, 233 SPRING ST, NEW YORK, NY 10013 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity |
| IDS Number: TK300 |
| ISSN: 0740-7750 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |