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| LIPOSOME-MEDIATED GENE-TRANSFER AND EXPRESSION VIA THE SKIN |
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| Author(s): ALEXANDER MY, AKHURST RJ |
| Source: HUMAN MOLECULAR GENETICS Volume: 4 Issue: 12 Pages: 2279-2285 Published: DEC 1995 |
| Times Cited: 83 References: 25 |
| Abstract: A beta-galactosidase gene expression construct was used to investigate the effectiveness of gene delivery and expression when DNA/liposome complexes were topically applied to mouse skin in vivo. DNA was complexed with a commercial preparation of N-[1-(2,3-dioleoyloxy) propyl]-N,N,N-trimethyl-ammoniummethyl-sulphate (DOTAP) in a ratio of 1:1.6 (w/w). The DNA rapidly penetrated the skin and was expressed in the epidermis, dermis and hair follicles. A DNA concentration of 267 mu g/ml DNA was found to be optimal for efficient transfection. Expression was seen as early as 6 h post-application, persisted at high levels 24 and 48 h post-treatment, but was markedly reduced by 7 days after application. In conclusion, utilising a commercially available liposome preparation, topically-applied DNA/liposome complexes can be efficiently delivered and expressed in several cell types within the skin. This simple, non-invasive technique may have implications for a number of gene therapy applications. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV GLASGOW, YORKHILL HOSP, DEPT MED GENET, GLASGOW G3 8SJ, LANARK SCOTLAND |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Genetics & Heredity |
| IDS Number: TK363 |
| ISSN: 0964-6906 |
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