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| ISWI, A MEMBER OF THE SW12/SNF2 ATPASE FAMILY, ENCODES THE 140-KDA SUBUNIT OF THE NUCLEOSOME REMODELING FACTOR |
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| Author(s): TSUKIYAMA T, DANIEL C, TAMKUN J, WU C |
| Source: CELL Volume: 83 Issue: 6 Pages: 1021-1026 Published: DEC 15 1995 |
| Times Cited: 254 References: 44 |
| Abstract: The generation of an accessible heat shock promoter in chromatin in vitro requires the concerted action of the GAGA transcription factor and NURF, an ATP-dependent nucleosome remodeling factor. NURF is composed of four subunits and is biochemically distinct from the SW12/SNF2 multiprotein complex, a transcriptional activator that also appears to alter nucleosome structure. We have obtained protein microsequence and immunological evidence identifying the 140 kDa subunit of NURF as ISWI, previously of unknown function but highly related to SW12/SNF2 only in the ATPase domain. The ISWI protein is localized to the cell nucleus and is expressed throughout Drosophila development at levels as high as 100,000 molecules/cell. The convergence of biochemical and genetic studies on ISWI and SW12/SNF2 underscores these ATPases and their close relatives as key components of independent systems for chromatin remodeling. |
| Document Type: Article |
| Language: English |
Addresses:
1. NCI, BIOCHEM LAB, BETHESDA, MD 20892 USA
2. UNIV CALIF SANTA CRUZ, DEPT BIOL, SANTA CRUZ, CA 95064 USA |
| Publisher: CELL PRESS, 50 CHURCH ST CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: TK745 |
| ISSN: 0092-8674 |
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