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| GENE-TRANSFER INTO THE MEDFLY, CERATITIS-CAPITATA, WITH A DROSOPHILA-HYDEI TRANSPOSABLE ELEMENT |
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| Author(s): LOUKERIS TG, LIVADARAS I, ARCA B, ZABALOU S, SAVAKIS C |
| Source: SCIENCE Volume: 270 Issue: 5244 Pages: 2002-2005 Published: DEC 22 1995 |
| Times Cited: 195 References: 23 |
| Abstract: Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly (medfly) Ceratitis capitata with a germline transformation system based on the transposable element Mines from Drosophila hydei. Transformants were identified as phenotypic revertants of a white-eyed mutation carried by the recipient strain. Clusters of transformants were detected among the progeny of 390 individuals screened for germline transformation. Five independent and phenotypically active integration events were identified, in each of which a single copy of the transposon was inserted into a different site of the medfly genome. Molecular analysis indicates that they represent transposase-mediated insertions of the transposon into medfly chromosomes. |
| Document Type: Article |
| Language: English |
Addresses:
1. FDN RES & TECHNOL HELLAS, INST MOLEC BIOL & BIOTECHNOL, GR-71110 CRETE, GREECE
2. UNIV CRETE, SCH MED, DIV BASIC MED SCI, CRETE, GREECE |
| Publisher: AMER ASSOC ADVAN SCIENCE, 1333 H ST NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: TL420 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |