| A packaging cell line for propagation of recombinant adenovirus vectors containing two lethal gene-region deletion |
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| Author(s): Wang Q, Jia XC, Finer MH |
| Source: GENE THERAPY Volume: 2 Issue: 10 Pages: 775-783 Published: DEC 1995 |
| Times Cited: 73 References: 37 |
| Abstract: A cell line that provides the E1 as well as the E4 gene functions of human adenovirus 5 has been established by introduction of the full-length Ad5 E4 region into 293 cells. To avoid the E1A transactivation of the E4 gene expression, the E4 promoter was replaced by the mouse alpha inhibin promoter containing a cAMP response element. This cell line was used to generate E1/E4-deleted adenovirus vectors containing a lacZ gene in the E1 region under the control of mouse pgk promoter. The titer and the lacZ gene expression of E1/E4-deleted adenovirus vector were comparable to those of E1-deleted vectors. Evidence of cytopathic effect was absent following infection of nonpermissive cell lines with E1/E4-deleted adenovirus in vitro. Establishment of the 293-E4 cell line and the generation of E1/E4-deleted adenovirus vectors may prolong gene expression in vivo and significantly improve the safety of adenovirus vectors for human gene therapy. |
| Document Type: Article |
| Language: English |
Addresses:
1. CELL GENESYS INC, FOSTER CITY, CA 94404 USA |
| Publisher: STOCKTON PRESS, HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS |
| Subject Category: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental |
| IDS Number: TL874 |
| ISSN: 0969-7128 |