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| Cyclin A-kinase regulation of E2F-1 DNA binding function underlies suppression of an S phase checkpoint |
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| Author(s): Krek W, Xu GF, Livingston DM |
| Source: CELL Volume: 83 Issue: 7 Pages: 1149-1158 Published: DEC 29 1995 |
| Times Cited: 273 References: 43 |
| Abstract: Commitment of mammalian cells to enter S phase enables the transcription factor E2F-1 to activate certain genes whose products mediate cell cycle advance. In S phase, E2F-1 forms stable complexes with cyclin A-kinase, which in turn eliminates E2F-1 DNA binding function. Here, we show that suppression of E2F-1 DNA-binding activity by cyclin A-kinase is linked to orderly S phase progression. Disruption of this linkage resulted in S phase delay/arrest followed by regrowth or apoptosis, depending upon whether the DNA-bound E2F-1 could transactivate. Hence, the unscheduled presence of E2F-1 on specific DNA sequences during S phase can activate a specific S phase checkpoint, thereby linking transcription, DNA replication, and cell cycle control. |
| Document Type: Article |
| Language: English |
Addresses:
1. DANA FARBER CANC INST, BOSTON, MA 02115 USA
2. HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: TM762 |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |