| | |  | | | | Record from Web of Science® | | | | | | |
| Identification and expression cloning of a leptin receptor, OB-R |
|
|
| Author(s): Tartaglia LA, Dembski M, Weng X, Deng NH, Culpepper J, Devos R, Richards GJ, Campfield LA, Clark FT, Deeds J, Muir C, Sanker S, Moriarty A, Moore KJ, Smutko JS, Mays GG, Woolf EA, Monroe CA, Tepper RI |
| Source: CELL Volume: 83 Issue: 7 Pages: 1263-1271 Published: DEC 29 1995 |
| Times Cited: 1,870 References: 36 |
| Abstract: The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [I-125]leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA expression library was prepared from mouse choroid plexus and screened with a leptin-AP fusion protein to identify a leptin receptor (OB-R). OB-R is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Genetic mapping of the gene encoding OB-R shows that it is within the 5.1 cM interval of mouse chromosome 4 that contains the db locus. |
| Document Type: Article |
| Language: English |
| Reprint Address: Tartaglia, LA (reprint author), MILLENNIUM PHARMACEUT INC, 640 MEM DR, CAMBRIDGE, MA 02139 USA |
Addresses:
1. ROCHE RES GENT, B-9000 GHENT, BELGIUM
2. F HOFFMANN LA ROCHE & CO LTD, CH-4002 BASEL, SWITZERLAND
3. HOFFMANN LA ROCHE INC, DEPT METAB DIS, NUTLEY, NJ 07110 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: TM762 |
| ISSN: 0092-8674 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |