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| V-src kinase shifts the cadherin-based cell adhesion from the strong to the weak state and beta catenin is not required for the shift |
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| Author(s): Takeda H, Nagafuchi A, Yonemura S, Tsukita S, Behrens J, Birchmeier W, Tsukita S |
| Source: JOURNAL OF CELL BIOLOGY Volume: 131 Issue: 6 Pages: 1839-1847 Part: Part 2 Published: DEC 1995 |
| Times Cited: 177 References: 47 |
| Abstract: The elevation of tyrosine phosphorylation level is thought to induce the dysfunction of cadherin through the tyrosine phosphorylation of beta catenin. We evaluated this assumption using two cell lines. First, using temperature-sensitive v-src-transfected MDCK cells, we analyzed the modulation of cadherin-based cell adhesion by tyrosine phosphorylation. Cell aggregation and dissociation assays at nonpermissive and permissive temperatures indicated that elevation of the tyrosine phosphorylation does not totally affect the cell adhesion ability of cadherin but shifts it from a strong to a weak state. The tyrosine phosphorylation levels of beta catenin, ZO-1, ERM (ezrin/radixin/moesin), but not alpha catenin, vinculin, and alpha-actinin, were elevated in the weak state. To evaluate the involvement of the tyrosine phosphorylation of beta catenin in this shift of cadherin-based cell adhesion, we introduced v-src kinase into L fibroblasts expressing the cadherin-alpha catenin fusion protein, in which beta catenin is not involved in cell adhesion. The introduction of v-src kinase in these cells shifted their adhesion from a strong to a weak state. These findings indicated that the tyrosine phosphorylation of beta catenin is not required for the strong-to-weak state shift of cadherin-based cell adhesion, but that the tyrosine phosphorylation of other junctional proteins, ERM, ZO-1 or unidentified proteins is involved.
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| Document Type: Article |
| Language: English |
Addresses:
1. KYOTO UNIV, FAC MED, DEPT CELL BIOL, SAKYO KU, KYOTO 606, JAPAN
2. NATL INST PHYSIOL SCI, DEPT INFORMAT PHYSIOL, CELL BIOL LAB, OKAZAKI, AICHI 444 JAPAN
3. GRAD UNIV ADV SCI, SCH LIFE SCI, DEPT PHYSIOL SCI, OKAZAKI, AICHI 444 JAPAN
4. KYOTO UNIV, COLL MED TECHNOL, SAKYO KU, KYOTO 606, JAPAN
5. MAX DELBRUCK CTR MOLEC MED, D-13122 BERLIN, GERMANY |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA |
| Subject Category: Cell Biology |
| IDS Number: TN760 |
| ISSN: 0021-9525 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |