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| Neuroglia and pioneer neurons express UNC-6 to provide global and local netrin cues for guiding migrations in C-elegans |
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| Author(s): Wadsworth WG, Bhatt H, Hedgecock EM |
| Source: NEURON Volume: 16 Issue: 1 Pages: 35-46 Published: JAN 1996 |
| Times Cited: 177 References: 31 |
| Abstract: Netrins are laminin-related proteins that guide circumferential migrations on the ectoderm. To understand how netrin cues direct cell movements, we examined the expression of nematode netrin UNC-6 from embryo to adult. UNC-6 is expressed in 12 types of neuroglia and neurons, creating a hierarchy of netrin cues in the developing nervous system. Comparing gene expression pattern with in vivo phenotypes, we suggest how multiple netrin cues, each with a characteristic role, guide cells and axons during development. We also present the molecular analysis of selective loss-of-function and null alleles. The results indicate that the biological activities of netrins are mediated through distinct protein domains. Subtle mutations in domain VI can produce selective defects in both direction- and tissue-specific guidance. EGF-like module V-2 is essential for dorsal guidance activity; we infer this module is important for interactions between UNC-6 and the dorsal guidance receptor UNC-5. |
| Document Type: Article |
| Language: English |
| Reprint Address: Wadsworth, WG (reprint author), ROBERT WOOD JOHNSON MED SCH, DEPT PATHOL, PISCATAWAY, NJ 08854 USA |
Addresses:
1. ROCHE INST MOLEC BIOL, NUTLEY, NJ 07110 USA
2. JOHNS HOPKINS UNIV, DEPT BIOL, BALTIMORE, MD 21218 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Neurosciences |
| IDS Number: TT307 |
| ISSN: 0896-6273 |
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