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| Antisense properties of duplex- and triplex-forming PNAs |
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| Author(s): Knudsen H, Nielsen PE |
| Source: NUCLEIC ACIDS RESEARCH Volume: 24 Issue: 3 Pages: 494-500 Published: FEB 1 1996 |
| Times Cited: 168 References: 40 |
| Abstract: The potential of peptide nucleic acids (PNAs) as specific inhibitors of translation has been studied, PNAs with a mixed purine/pyrimidine sequence form duplexes, while homopyrimidine PNAs form (PNA)(2)/RNA triplexes with complementary sequences on RNA, We show here that neither of these PNA/RNA structures are substrates for RNase H. Translation experiments performed in cell-free extracts showed that a 15mer duplex-forming PNA blocked translation in a dose-dependent manner when the target was 5-proximal to the AUG start codon on the RNA, whereas similar 10-, 15- or 20mer PNAs had no effect when targeted towards sequences in the coding region. Tripler-forming 10mer PNAs were efficient and specific antisense agents with a target overlapping the AUG start codon and caused arrest of ribosome elongation with a target positioned in the coding region of the mRNA, Furthermore, translation could be blocked with a 6mer bisPNA or with a clamp PNA, forming partly a tripler, partly a duplex, with its target sequence in the coding region of the mRNA. |
| Document Type: Article |
| Language: English |
Addresses:
1. PANUM INST, BIOCHEM LAB B, DEPT MED BIOCHEM & GENET, CTR BIOMOLEC RECOGNIT, DK-2200 COPENHAGEN N, DENMARK |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: TV169 |
| ISSN: 0305-1048 |
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