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| Simultaneous determination of ethylene glycol and its major toxic metabolite, glycolic acid, in serum by gas chromatography |
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| Author(s): Yao HH, Porter WH |
| Source: CLINICAL CHEMISTRY Volume: 42 Issue: 2 Pages: 292-297 Published: FEB 1996 |
| Times Cited: 24 References: 37 |
| Abstract: We developed a gas-chromatographic procedure for the simultaneous determination of ethylene glycol (EG) and its major toxic metabolite, glycolic acid (GA), suitable for clinical use in instances of EG intoxication, After serum protein precipitation with acetonitrile (containing internal standard), the supernate is treated with 2,2-dimethoxypropane (containing dimethylformamide) to remove water, and the volume is then reduced by evaporation to <100 mu L of dimethylformamide (but not to dryness). After trimethylsilyl derivatization, the resulting derivatives are analyzed by capillary column gas chromatography, Only 100 mu L of serum is required and the entire determination, including calibrators and controls, takes <2 h, The method gives a linear response to at least 10 g/L EG and 5 g/L GA and has a limit of detection <10 mg/L. Intraassay CV is less than or equal to 2.8% for EG (100 and 1000 mg/L) and GA (100 and 500 mg/L); between-day CV is less than or equal to 6.5%. The absolute recovery from serum was 91% for EG and 77-82% for GA (200 and 2000 mg/L each), Relative to calibrators prepared bovine serum albumin (70 g/L), the recovery was 99-104% for EG (100-5000 mg/L) and 95-105% for GA (50-2500 mg/L). No clinically important interference was detected for >60 exogenous or endogenous compounds and drugs. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV KENTUCKY, MED CTR, DEPT PATHOL & LAB MED, LEXINGTON, KY 40536 USA |
| Publisher: AMER ASSOC CLINICAL CHEMISTRY, 2101 L STREET NW, SUITE 202, WASHINGTON, DC 20037-1526 |
| Subject Category: Medical Laboratory Technology |
| IDS Number: TV950 |
| ISSN: 0009-9147 |
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