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| A radiation hybrid map of the human genome |
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| Author(s): Gyapay G, Schmitt K, Fizames C, Jones H, VegaCzarny N, Spillett D, Muselet D, PrudHomme JF, Dib C, Auffray C, Morissette J, Weissenbach J, Goodfellow PN |
| Source: HUMAN MOLECULAR GENETICS Volume: 5 Issue: 3 Pages: 339-346 Published: MAR 1996 |
| Times Cited: 405 References: 15 |
| Abstract: We have developed a panel of whole-genome radiation hybrids by fusing irradiated diploid human fibroblasts with recipient hamster cells. This panel of -168 cell lines has been typed with microsatellite markers of known genetic location, Of 711 AFM genetic markers 404 were selected to construct a robust framework map that spans all the autosomes and the X chromosome. To demonstrate the utility of the panel, 374 expressed sequence tags (ESTs) previously assigned to chromosomes 1, 2, 14 and 16 were localized on this map. All of these ESTs could be positioned by pairwise linkage to one of the framework markers with a LOD score of greater than 8. The whole genome radiation hybrid panel described here has been used as the starting material for the Genebridge4 panel that is being made widely available for genome mapping projects. |
| Document Type: Article |
| Language: English |
| Reprint Address: Gyapay, G (reprint author), GENETHON, CNRS URA 1922, 1 RUE INT, F-91000 EVRY, FRANCE |
Addresses:
1. UNIV CAMBRIDGE, DEPT GENET, CAMBRIDGE, ENGLAND
2. GENEXPRESS, F-94801 VILLEJUIF, FRANCE
3. GENETHON, F-94801 VILLEJUIF, FRANCE
4. CNRS UPR 420, F-94801 VILLEJUIF, FRANCE
5. CHUL, ST FOY, PQ G1V 4G2 CANADA |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Genetics & Heredity |
| IDS Number: TY879 |
| ISSN: 0964-6906 |
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