| | |  | | | | Record from Web of Science® | | | | | | |
| Protein folding triggered by electron transfer |
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| Author(s): Pascher T, Chesick JP, Winkler JR, Gray HB |
| Source: SCIENCE Volume: 271 Issue: 5255 Pages: 1558-1560 Published: MAR 15 1996 |
| Times Cited: 245 References: 32 |
| Abstract: Rapid photochemical electron injection into unfolded ferricytochrome c titrated with 2.3 to 4.6 M guanidine hydrochloride (GuHCl) at pH 7 and 40 degrees C produced unfolded ferrocytochrome, which then converted to the folded protein. Two folding phases were observed: a fast process with a time constant of 40 microseconds (4.6 M GuHCl), and a slower phase with a rate constant of 90 +/- 20 per second (2.3 M GuHCl). The activation free energy for the slow step varied linearly with GuHCl concentration; the rate constant, extrapolated to aqueous solution, was 7600 per second, Electron-transfer methods can bridge the nanosecond to millisecond measurement time gap for protein folding. |
| Document Type: Article |
| Language: English |
Addresses:
1. CALTECH, BECKMAN INST, PASADENA, CA 91125 USA
2. HAVERFORD COLL, HAVERFORD, PA 19041 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: TZ983 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |