| | |  | | | | Record from Web of Science® | | | | | | |
| Tetrahymena histone acetyltransferase A: A homolog to yeast Gcn5p linking histone acetylation to gene activation |
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| Author(s): Brownell JE, Zhou JX, Ranalli T, Kobayashi R, Edmondson DG, Roth SY, Allis CD |
| Source: CELL Volume: 84 Issue: 6 Pages: 843-851 Published: MAR 22 1996 |
| Times Cited: 791 References: 59 |
| Abstract: We report the cloning of a transcription-associated histone acetyltransferase type A (HAT A). This Tetrahymena enzyme is strikingly homologous to the yeast protein Gcn5, a putative transcriptional adaptor, and we demonstrate that recombinant Gcn5p possesses HAT activity. Both the ciliate enzyme and Gcn5p contain potential active site residues found in other acetyltransferases and a highly conserved bromodomain. The presence of this domain in nuclear A-type HATs, but not in cytoplasmic B-type HATs, suggests a mechanism whereby HAT A is directed to chromatin to facilitate transcriptional activation. These findings shed light on the biochemical function of the evolutionarily conserved Gcn5p-Ada complex, directly linking histone acetylation to gene activation, and indicate that histone acetylation is a targeted phenomenon. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV ROCHESTER, DEPT BIOL, ROCHESTER, NY 14627 USA
2. COLD SPRING HARBOR LAB, COLD SPRING HARBOR, NY 11724 USA
3. UNIV TEXAS, MD ANDERSON CANC CTR, DEPT BIOCHEM & MOLEC BIOL, HOUSTON, TX 77030 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: UC381 |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |