| | |  | | | | Record from Web of Science® | | | | | | |
| Karyotyping human chromosomes by combinatorial multi-fluor FISH |
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| Author(s): Speicher MR, Ballard SG, Ward DC |
| Source: NATURE GENETICS Volume: 12 Issue: 4 Pages: 368-375 Published: APR 1996 |
| Times Cited: 684 References: 31 |
| Abstract: We have developed epifluorescence filter sets and computer software for the detection and discrimination of 27 different DNA probes hybridized simultaneously. For karyotype analysis, a pool of human chromosome painting probes, each labelled with a different fluor combination, was hybridized to metaphase chromosomes prepared from normal cells, clinical specimens, and neoplastic cell lines. Both simple and complex chromosomal rearrangements could be detected rapidly and unequivocally; many of the move complex chromosomal abnormalities could not be delineated by conventional cytogenetic banding techniques. Our data suggest that multiplex-fluorescence in situ hybridization (M-FISH) could have wide clinical utility and complement standard cytogenetics, particularly for the characterization of complex karyotypes. |
| Document Type: Article |
| Language: English |
| Reprint Address: Speicher, MR (reprint author), YALE UNIV, SCH MED, DEPT GENET & MOL BIOPHYS, 333 CEDAR ST, NEW HAVEN, CT 06510 USA |
Addresses:
1. YALE UNIV, SCH MED, DEPT BIOCHEM, NEW HAVEN, CT 06510 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: UC553 |
| ISSN: 1061-4036 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |