| | |  | | | | Record from Web of Science® | | | | | | |
| Assignment of psoriasin to human chromosomal band 1q21: Coordinate overexpression of clustered genes in psoriasis |
|
|
| Author(s): Hardas BD, Zhao XP, Zhang J, Xia LQ, Stoll S, Elder JT |
| Source: JOURNAL OF INVESTIGATIVE DERMATOLOGY Volume: 106 Issue: 4 Pages: 753-758 Published: APR 1996 |
| Times Cited: 59 References: 42 |
| Abstract: Psoriasin is an abundant low molecular weight protein in keratinocytes fi om psoriatic lesions. Because of similarities in gene structure and expression to other genes on human chromosomal band 1q21, we hypothesized that psoriasin might also map to this region. To test this hypothesis, we identified and used a genomic lambda clone (lambda 9.2) as a probe for fluorescent in situ hybridization. lambda 9.2 detected the 1q21 region in 81% of 52 chromosomes 1 examined, although it also hybridized to acrocentric chromosomes. lambda 9.2 DNA yielded polymerase chain reaction amplification of a 121-bp sequence colinear with psoriasin cDNA, as did genomic DNA from hybrid cell lines containing all or part of chromosome 1. The psoriasin gene was present on a 380-kb yeast artificial chromosome clone that was previously mapped to 1q21 and shown to contain calcyclin; here it is also shown to contain MRP8 and CaN19. Psoriasin and several other tightly linked 1q21 genes were markedly overexpressed in psoriatic lesions, suggesting a role for these clustered genes in the regulation of epidermal proliferation. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV MICHIGAN, DEPT DERMATOL, ANN ARBOR, MI 48109 USA
2. UNIV MICHIGAN, DEPT RADIAT ONCOL, ANN ARBOR, MI 48109 USA
3. UNIV MICHIGAN, DEPT HUMAN GENET, ANN ARBOR, MI 48109 USA |
| Publisher: BLACKWELL SCIENCE INC, 238 MAIN ST, CAMBRIDGE, MA 02142 |
| Subject Category: Dermatology |
| IDS Number: UC787 |
| ISSN: 0022-202X |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |