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| Fluorescence in situ hybridization for detecting ErbB-2 amplification in breast tumor fine needle aspiration biopsies |
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| Author(s): Sauter G, Feichter G, Torhorst J, Moch H, Novotna H, Wagner U, Durmuller U, Waldman FM |
| Source: ACTA CYTOLOGICA Volume: 40 Issue: 2 Pages: 164-173 Published: MAR-APR 1996 |
| Times Cited: 43 References: 27 |
| Abstract: OBJECTIVE: To evaluate the feasibility of erbB-2 amplification? analysis of fine needle aspiration (FNA) biopsies. STUDY DESIGN: FNA smears and dissociated nuclei from 58 breast cancer samples were examined by dual-labeling fluorescence in situ hybridization (FISH) with probes for centromere 17 and the erbB-2 gene. The results were compared with the outcome of erbB-2 immunohistochemistry.
RESULTS: Tumors were categorized according to the erbB-2/centromere 17 signal ratio. There were 23 tumors with high-level amplification, four cases with a low-level erbB-2 gain and 27 tumors with normal erbB-2 content. Four tumors showed an erbB-2 deletion, all in patients less than or equal to 42 years of age. ErbB-2 amplification was strongly associated with positive erbB-2 immunostaining (P < .0001). Comparison of FISH analysis on dissociated cells and on FNA biopsies showed high correspondence (P < .0001).
CONCLUSION: FISH allows reliable detection of erbB-2 gene amplification on FNA biopsies.
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| Document Type: Article |
| Language: English |
| Reprint Address: Sauter, G (reprint author), UNIV BASEL, DEPT PATHOL, SCHONBEINSTR 40, CH-4003 BASEL, SWITZERLAND |
Addresses:
1. UNIV SAN FRANCISCO, DEPT LAB MED, DIV MOLEC CYTOMET, SAN FRANCISCO, CA 94117 USA
2. UNIV BASEL, INST PATHOL, DIV GYNECOL CYTOL, BASEL, SWITZERLAND
3. UNIV BASEL, INST PATHOL, LAB MOLEC CYTOGENET, BASEL, SWITZERLAND |
| Publisher: SCI PRINTERS & PUBL INC, P.O. DRAWER 12425 8342 OLIVE BLVD, ST LOUIS, MO 63132 |
| Subject Category: Cell Biology; Pathology |
| IDS Number: UD400 |
| ISSN: 0001-5547 |
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