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| Increased cyclooxygenase-2 levels in carcinogen-induced rat colonic tumors |
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| Author(s): DuBois RN, Radhika A, Reddy BS, Entingh AJ |
| Source: GASTROENTEROLOGY Volume: 110 Issue: 4 Pages: 1259-1262 Published: APR 1996 |
| Times Cited: 338 References: 31 |
| Abstract: Background & Aims: Multiple studies show that continuous use of nonsteroidal anti-inflammatory drugs (NSAIDs) lowers the risk of colon cancer in humans and carcinogen-treated rodents. One target for NSAIDs is cyclooxygenase (COX), and two isoforms of this enzyme have been identified: COX-1 and COX-2. The present study was undertaken to determine if there is differential expression of COX in colonic tumors in azoxymethane-treated rats. Methods: COX-1 and COX-2 messenger RNA levels were determined by Northern blot analysis of total RNA isolated from colonic tumors and normal adjacent mucosa. COX-2 protein levels were determined by Western blotting analysis. Quantitation of relative band densities was performed using standard densitometry scanning techniques. Results: There was a marked increase in COX-2 RNA levels in six of six of the colonic tumors compared with paired normal mucosa. In contrast, there was equivalent intensity of the COX-1 RNA transcript between the normal mucosa and tumor in all of the specimens examined. Western blotting analysis showed an increase in the level of the COX-2 protein in four of five of the colonic tumor samples. Conclusions: COX-2 but not COX-1 gene expression is markedly elevated in most colonic tumors examined in azoxymethane-treated rodents. COX-2 may provide a target for chemopreventive strategies for colorectal cancer. |
| Document Type: Article |
| Language: English |
| Reprint Address: DuBois, RN (reprint author), VANDERBILT UNIV, MED CTR, DEPT MED, GI MCN C-2104, NASHVILLE, TN 37232 USA |
Addresses:
1. VANDERBILT UNIV, MED CTR, DEPT CELL BIOL, NASHVILLE, TN USA
2. AMER HLTH FDN, VALHALLA, NY 10595 USA |
| Publisher: W B SAUNDERS CO, INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 |
| Subject Category: Gastroenterology & Hepatology |
| IDS Number: UD846 |
| ISSN: 0016-5085 |
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