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| Intracellular antibody against erbB-2 mediates targeted tumor cell eradication by apoptosis |
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| Author(s): Deshane J, Grim J, Loechel S, Siegal GP, Alvarez RD, Curiel DT |
| Source: CANCER GENE THERAPY Volume: 3 Issue: 2 Pages: 89-98 Published: MAR-APR 1996 |
| Times Cited: 54 References: 49 |
| Abstract: Methods were developed to achieve targeted eradication of the erbB-2 oncoprotein using gene constructs encoding anti-erbB-2 intracellular single-chain antibodies. This method of genetic intervention caused a marked cytocidal effect in erbB-2-overexpressing human ovarian tumor cells. Evaluation of the mechanistic basis of this phenomenon demonstrated that programmed cell death had been induced. Significantly, no cytocidal effect was observed in non-erbB-2-overexpressing tumors. The induction of apoptosis could be shown to be secondary to the intracellular antibody-mediated ectopic localization of the erbB-2 oncoprotein. Thus, the strategy of selective oncogene ''knock-out'' using intracellular antibodies represents a novel anticancer gene therapy strategy that offers the potential to achieve highly specific, targeted eradication oi human tumor cells. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV ALABAMA, GENE THERAPY PROGRAM, BIRMINGHAM, AL 35294 USA
2. UNIV ALABAMA, DEPT PATHOL, BIRMINGHAM, AL 35294 USA
3. UNIV ALABAMA, DEPT CELL BIOL, BIRMINGHAM, AL 35294 USA
4. UNIV ALABAMA, DEPT SURG, BIRMINGHAM, AL 35294 USA
5. UNIV ALABAMA, DEPT OBSTET & GYNECOL, BIRMINGHAM, AL 35294 USA |
| Publisher: APPLETON & LANGE, 25 VAN ZANT ST, E NORWALK, CT 06855 |
| Subject Category: Biotechnology & Applied Microbiology; Oncology; Genetics & Heredity; Medicine, Research & Experimental |
| IDS Number: UE286 |
| ISSN: 0929-1903 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |