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| Metabolites of 4-n-nonylphenol in wheat cell suspension cultures |
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| Author(s): Bokern M, Nimtz M, Harms HH |
| Source: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Volume: 44 Issue: 4 Pages: 1123-1127 Published: APR 1996 |
| Times Cited: 20 References: 25 |
| Abstract: 4-Nonylphenol, a metabolite of nonionic surfactants, was examined for its metabolism in cell cultures of wheat according to a standardized method. Four major radioactively labeled fractions were detected and isolated by chromatography on Sephadex LH-20 and semipreparative HPLC. Hydrolysis of the peaks resulted in two compounds which were purified by semipreparative HPLC. Using enzymatic hydrolysis, HPLC, GC-MS, and ESI-MS/MS, the chemical structures were elucidated. They were shown to be 4-(hydroxy)- and 4-(dihydroxy)nonylphenols which were glucosylated at the phenolic OH-group and further glucosylated, glucuronidated, and acylated with acetic acid or malonic acid. Except for C-1 and C-9, the hydroxylations occurred at all C atoms of the alkyl side chain. |
| Document Type: Article |
| Language: English |
| Reprint Address: Bokern, M (reprint author), BUNDESFORSCH ANSTALT LANDWIRTSCHAFT, INST PFLANZENERNAHRUNG & BODENKUNDE, BUNDESALLEE 50, D-38116 BRAUNSCHWEIG, GERMANY |
Addresses:
1. GESELL BIOTECHNOL FORSCH MBH, D-38124 BRAUNSCHWEIG, GERMANY |
| Publisher: AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 |
| Subject Category: Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology |
| IDS Number: UF776 |
| ISSN: 0021-8561 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |