| | |  | | | | Record from Web of Science® | | | | | | |
| Circumventing genetic restriction of protection against malaria with multigene DNA immunization: CD8(+) T cell-, interferon gamma-, and nitric oxide-dependent immunity |
|
|
| Author(s): Doolan DL, Sedegah M, Hedstrom RC, Hobart P, Charoenvit Y, Hoffman SL |
| Source: JOURNAL OF EXPERIMENTAL MEDICINE Volume: 183 Issue: 4 Pages: 1739-1746 Published: APR 1 1996 |
| Times Cited: 197 References: 50 |
| Abstract: Despite efforts to develop vaccines that protect against malaria by inducing CD8(+) T cells that kill infected hepatocytes, no subunit vaccine has been shown to circumvent the genetic restriction inherent in this approach, and little is known about the interaction of subunit vaccine-induced immune effectors and infected hepatocytes. We now report that immunization with plasmid DNA encoding the Plasmodium yoelii circumsporozoite protein protected one od five strains of mice against malaria (H-2(d), 75%); a PyHEP17 DNA vaccine protected three of the five strains (H-2(a), 71%; H-2(k), 54%; H-2(d), 26%); and the combination protected 82% of H-2(a), 90% of H-2(k), and 88% of H-2(d) mice. Protection was absolutely dependent on CD8(+) T cells, IFN-gamma, or nitric oxide. These data introduce a new target of protective preerythrocytic immune responses, PyHEP17 and its P. falciparum homologue, and provide a realistic perspective on the opportunities and challenges inherent in developing malaria vaccines that target the infected hepatocyte. |
| Document Type: Article |
| Language: English |
Addresses:
1. USN, MED RES INST, MALARIA PROGRAM, BETHESDA, MD 20889 USA
2. UNIV MARYLAND, BALTIMORE, MD 21201 USA
3. VICAL INC, SAN DIEGO, CA 92121 USA |
| Publisher: ROCKEFELLER UNIV PRESS, 222 E 70TH STREET, NEW YORK, NY 10021 |
| Subject Category: Immunology; Medicine, Research & Experimental |
| IDS Number: UH144 |
| ISSN: 0022-1007 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |