| Prostatic corpora amylacea and crystalloids: Similarities and difference on ultrastructural and histochemical studies |
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| Author(s): Drachenberg CB, Papadimitriou JC |
| Source: JOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY Volume: 28 Issue: 2 Pages: 141-150 Published: APR 1996 |
| Times Cited: 9 References: 34 |
| Abstract: Intraluminal contents in benign and malignant prostate glands from 10 prostatectomies mere studied by light and electron microscopy as as well as X-ray microanalysis. Ultrastructural immunolocalization of keratin and analysis of the pattern of lectin binding for wheat germ agglutinin (WGA), peanut agglutinin (PNA) and soy bean agglutinin (SBA) mere performed. By electron microscopy corpora amylacea were composed of bundles of fibrils and occasional interspersed electron-dense areas. Crystalloids on the other hand were relatively electron-dense formations without any identifiable substructure. Complete or partial enclose ment of the crystalloids by the fibrillary or electron-dense material that forms the corpora amylacea was often seen. Histochemistry localized keratin and glycoproteins in all types of intraluminal contents. However, the proportion of these components varied. Keratin and WGA binding were identified primarily in the amorphous secretions and in corpora amylacea, but were only minimally represented in crystalloids. PNA and SBA were found predominantly in crystalloids, with only minimal amounts identified in corpora amylacea. By X-ray microanalysis sulfur was identified primarily in crystalloids and surrounding amorphous secretion, but lesser quantities of sulfur were also found in corpora amylacea. In summary the morphological and histochemical findings indicate that the intraluminal contents in benign and malignant glands form a continuous spectrum and are largely composed of material derived from the components of lining cells. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV MARYLAND, SCH MED, DEPT PATHOL, BALTIMORE, MD 21201 USA |
| Publisher: EDITRICE COMPOSITORI BOLOGNA, VIA STALINGRADO 97/2, I-40128 BOLOGNA, ITALY |
| Subject Category: Cell Biology; Pathology |
| IDS Number: UJ393 |
| ISSN: 1122-9497 |