| | |  | | | | Record from Web of Science® | | | | | | |
| Immunohistochemical p53 staining is of limited value in the staging and prognostic prediction of colorectal cancer |
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| Author(s): Kressner U, Lindmark G, Gerdin B, Pahlman L, Glimelius B |
| Source: ANTICANCER RESEARCH Volume: 16 Issue: 2 Pages: 951-957 Published: MAR-APR 1996 |
| Times Cited: 37 References: 39 |
| Abstract: Purpose: To compare immunohistochemical staining using different anti-p53 antibodies, and to evaluate the possible clinical implications of the overexpression cf p53 in a series of patients resected for colorectal cancer with a long follow-rip. Methods. Tumor biopsy samples were collected from 294 surgical colorectal cancer specimens, obtained from two series of patients with a median follow-rip of 4.5 years. The samples stained with four commercially available anti-p53 antibodies, (mouse monoclonal antibodies 421, 1801, and DO-7; and rabbit polyclonal antibody CMI), were evaluated and compared with cryosections from a subset of 20 biopsies from tumors iii various stages and grades, obtained from patients with different outcomes. Results: DO-7 gave a homogeneous nuclear staining, which, when further investigated, turned out to be identical in the formalin-fixed paraffin-embedded and ill the frozen specimens. Therefore, DO-7 was found to be suitable Sol the further analysis of archival or frozen sections from the sample. p53 overexpression was shown in 162 (55%) cases, with a significantly higher proportion having DNA aneuploidy (p<0.01) in left-sided colonic and rectal tumors (p<0.01). p53 staining was not associated with tumor stage, tumor grade, or survival. Conclusions: A significantly higher: proportion of p53 overexpressing tumors are DNA aneuploid, indicating that mutations in the TP53 gene constitute a sign of generic instability, which might be of importance in malignant transformation. However, we could not Sind any indication that TP53 mutations, as reflected in the overexpression of p53, constitute a prerequisite for tumor progression in colorectal cancer. |
| Document Type: Article |
| Language: English |
| Reprint Address: Kressner, U (reprint author), AKAD SJUKHUSET, DEPT SURG, S-75185 UPPSALA, SWEDEN |
Addresses:
1. UNIV UPPSALA HOSP, DEPT PLAST SURG, S-75185 UPPSALA, SWEDEN
2. UNIV UPPSALA HOSP, DEPT ONCOL, S-75185 UPPSALA, SWEDEN |
| Publisher: INT INST ANTICANCER RESEARCH, EDITORIAL OFFICE 1ST KM KAPANDNTIOU-KALAMOU RD KAPANDRITI, POB 22, ATHENS 19014, GREECE |
| Subject Category: Oncology |
| IDS Number: UP717 |
| ISSN: 0250-7005 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |