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| The transcription activator steroidogenic factor-1 is preferentially expressed in the human pituitary gonadotroph |
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| Author(s): Asa SL, Bamberger AM, Cao B, Wong M, Parker KL, Ezzat S |
| Source: JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM Volume: 81 Issue: 6 Pages: 2165-2170 Published: JUN 1996 |
| Times Cited: 59 References: 29 |
| Abstract: Steroidogenic factor-1 (SF-1), also known as adrenal-4-binding protein, is a transcription factor thai; is important for the differentiation of steroidogenic tissues. We investigated whether SE-1 is expressed in specific hormone-producing cell types in the human pituitary and its adenomas. Pituitary adenomas (n = 35) were collected at the time of surgery, and normal adenohypophyses were obtained from autopsies; 3 corticotroph adenomas were excluded because pit-1 messenger ribonucleic acid (mRNA) expression indicated contamination by nontumorous elements. Expression of SF-1 mRNA was determined by reverse transcription-PCR. SF-1 protein was localized with immuno-cytochemistry. By reverse transcription-PCR, SE-1 mRNA was found in the nontumorous pituitary and in pituitary adenomas expressing gonadotropins. All 8 gonadotroph adenomas had a strong signal for SF-1. SE-1 mRNA was also detected in 2 of 3 corticotroph adenomas, 2 of 13 somatotroph/mammosomatotroph adenomas, 1 of 6 lactotroph adenomas, and 2 silent subtype 3 adenomas; however, in most of the positive tumors there was also positivity for FSH beta and/or LH beta mRNA, suggesting that contaminating nontumorous gonadotrophs may be the source of SF-1 mRNA signal. SF-1 protein was localized by immunocytochemistry in the nuclei of scattered cells of the nontumorous adenohypophysis that were shown to be gonadotrophs with double immunostaining and in gonadotroph adenomas; nuclear staining was not found in other adenoma types, except in areas shown to contain trapped nontumorous tissue. We conclude that SF-1 expression correlates with the expression of gonadotropins. These findings implicate SF-l as a cell-specific transcription factor that may regulate gonadotroph differentiation in the pituitary.
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| Document Type: Article |
| Language: English |
| Reprint Address: Asa, SL (reprint author), MT SINAI HOSP, DEPT PATHOL, 600 UNIV AVE, TORONTO, ON M5G 1X5 CANADA |
Addresses:
1. UNIV TORONTO, WELLESLEY HOSP, DEPT MED ENDOCRINOL, TORONTO, ON M5G 1X5 CANADA
2. UNIV HAMBURG, INST HORMONE & FERTIL RES, HAMBURG, GERMANY
3. DUKE UNIV, MED CTR, HOWARD HUGHES MED INST, DEPT MED & BIOCHEM, DURHAM, NC 27705 USA |
| Publisher: ENDOCRINE SOC, 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA |
| Subject Category: Endocrinology & Metabolism |
| IDS Number: UQ022 |
| ISSN: 0021-972X |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |