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| Isolation and characterization of genes associated with chromosome-6 mediated tumor suppression in human malignant melanoma |
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| Author(s): Ray ME, Su YA, Meltzer PS, Trent JM |
| Source: ONCOGENE Volume: 12 Issue: 12 Pages: 2527-2533 Published: JUN 20 1996 |
| Times Cited: 38 References: 21 |
| Abstract: Melanocytic transformation is thought to occur by the sequential accumulation of genetic alterations, Evidence implicating human chromosome-6 as a site for a gene(s) involved in melanoma suppression comes from studies of LOH [loss of heterozygosity], cytogenetics and biologic reversion of tumorigenicity following the introduction of a normal chromosome 6 by microcell-mediated chromosome transfer (Trent et al., 1990), Using a tumorigenic melanoma cell line (UACC 903) and a chromosome-6 suppressed melanoma subline [UACC 903 (+6)], we have isolated a series of genes uniquely expressed in the suppressed subline, A modified PCR-based cDNA subtraction technique was used to generate subtracted cDNA sublibraries for both the parental and (+6) suppressed cells, A total of 32 randomly selected clones from the suppressed sublibrary were isolated and examined, with 24 detecting a transcript by Northern analysis, Of these 24 clones, 21 (88%) demonstrated elevated expressed by Northern analysis in the suppressed subline relative to the tumorigenic parental cell line, In 6/21 differentially expressed clones (29%), expression was exclusive to the suppressed subline, Partial sequence analysis and database searching of these clones indicated that 5/6 were novel with one representing a previously characterized gene, Chromosomal localization of the five novel clones was performed following PCR amplification of a human/rodent somatic cell hybrid mapping panel or fluorescent in situ hybridization, One cDNA (termed AIM1) was localized to a band-region of chromosome 6 frequently deleted in melanomas (6q21), This novel approach should facilitate the identification of genes whose expression is causally related to the suppressed phenotype. |
| Document Type: Article |
| Language: English |
Addresses:
1. NIH, NATL CTR HUMAN GENOME RES, CANC GENET LAB, BETHESDA, MD 20892 USA
2. UNIV MICHIGAN, SCH MED, DEPT HUMAN GENET, ANN ARBOR, MI 48109 USA |
| Publisher: STOCKTON PRESS, HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS |
| Subject Category: Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity |
| IDS Number: UW487 |
| ISSN: 0950-9232 |
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