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| The Kaposi sarcoma-associated herpesvirus (KSHV) is present as an intact latent genome in KS tissue but replicates in the peripheral blood mononuclear cells of KS patients |
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| Author(s): Decker LL, Shankar P, Khan G, Freeman RB, Dezube BJ, Lieberman J, ThorleyLawson DA |
| Source: JOURNAL OF EXPERIMENTAL MEDICINE Volume: 184 Issue: 1 Pages: 283-288 Published: JUL 1 1996 |
| Times Cited: 158 References: 19 |
| Abstract: Short DNA sequences have been identified, originally in association with Kaposi's sarcoma (KS) biopsies, that are highly homologous to oncogenic, lymphotropic herpesviruses. Recently a virus, Kaposi sarcoma associated herpesvirus (KSHV) or human herpesvirus-8 (HHV-8), bearing these sequences has been identified in a cell line derived from a body cavity-based lymphoma. In this report, we show that the same sequences are present ill KS biopsies as DNA molecules of a form and size characteristic of latent herpesviruses-large, covalently closed, circular episomes. The genomes migrate with an apparent size larger than the herpesvirus Epstein-Barr virus (172 kb). This form of the viral genome was found in four of four biopsies and three of five peripheral blood samples from KS patients. Linear forms of the viral genome, characteristic of viral replication, were not detected in the biopsies, but were present ill the peripheral blood of three out of five patients. The sequences for KSHV/HHV-8 were also detected ill the blood of four of five allograft patients and three of five healthy donors without KS suggesting that the virus is widespread throughout the human population. |
| Document Type: Article |
| Language: English |
Addresses:
1. TUFTS UNIV, SCH MED, DEPT PATHOL, BOSTON, MA 02111 USA
2. HARVARD UNIV, SCH MED, CTR BLOOD RES, BOSTON, MA 02115 USA
3. TUFTS UNIV NEW ENGLAND MED CTR, DEPT SURG, BOSTON, MA 02111 USA
4. HARVARD UNIV, BETH ISRAEL HOSP, SCH MED, DIV HEMATOL ONCOL, BOSTON, MA 02215 USA |
| Publisher: ROCKEFELLER UNIV PRESS, 222 E 70TH STREET, NEW YORK, NY 10021 |
| Subject Category: Immunology; Medicine, Research & Experimental |
| IDS Number: UW677 |
| ISSN: 0022-1007 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |