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| A strategy for rapid and efficient DNA sequencing by mass spectrometry |
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| Author(s): Koster H, Tang K, Fu DJ, Braun A, vandenBoom D, Smith CL, Cotter RJ, Cantor CR |
| Source: NATURE BIOTECHNOLOGY Volume: 14 Issue: 9 Pages: 1123-1128 Published: SEP 1996 |
| Times Cited: 86 References: 37 |
| Abstract: Two methods of solid-phase Sanger DNA sequencing followed by detection with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry are demonstrated. In one method, sequencing ladders generated on an immobilized synthetic template were resolved up to the 63-mer including the primer Detection sensivity and resolution were sufficient for sequence analysis in the given range, This approach is particularly suitable for comparative (diagnostic) DNA sequencing. A second method that has the potential for high throughput de novo DNA sequencing is also presented; it uses immobilized duplex probes with five-base single-stranded overhangs to capture an unknown DNA template serving as primers for Sanger DNA sequencing. The power of mass spectrometry is demonstrated not only by its very high speed, but also by its ability to identify sequences that are not readable using gel electrophoresis. |
| Document Type: Article |
| Language: English |
| Reprint Address: Koster, H (reprint author), UNIV HAMBURG, DEPT BIOCHEM & MOL BIOL, MARTIN LUTHER KING PL 6, D-20146 HAMBURG, GERMANY |
Addresses:
1. SEQUENOM INC, BOSTON, MA 02110 USA
2. SEQUENOM INSTRUMENTS GMBH, D-22761 HAMBURG, GERMANY
3. JOHNS HOPKINS UNIV, SCH MED, DEPT PHARMACOL, BALTIMORE, MD 21205 USA
4. BOSTON UNIV, CTR ADV BIOTECHNOL, BOSTON, MA 02215 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Biotechnology & Applied Microbiology |
| IDS Number: VF843 |
| ISSN: 1087-0156 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |