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| IL-12 induces human T cells secreting IL-10 with IFN-gamma |
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| Author(s): Windhagen A, Anderson DE, Carrizosa A, Williams RE, Hafler DA |
| Source: JOURNAL OF IMMUNOLOGY Volume: 157 Issue: 3 Pages: 1127-1131 Published: AUG 1 1996 |
| Times Cited: 102 References: 22 |
| Abstract: A clear differentiation of Th1 and Th2 cytokine-secreting subsets in humans has not yet been defined, To further examine cytokine-directed differentiation of human T cell responses to both exogenous and autoantigens, we generated 346 short term T cell lines at limiting dilutions from six normal individuals to tetanus toroid and myelin basic protein in the presence of IL-2 with or without the addition of IL-12 and anti-IL-4 mAb. T cell lines were examined for [H-3]thymidine incorporation and cytokine secretion of IFN-gamma, IL-4, and IL-10. After culture in the presence of IL-12 and anti-IL-4 mAb, the predominant T cell response to Ag stimulation was simultaneous secretion of IL-10 and IFN-gamma. The concomitant secretion of IL-10 and IFN-gamma by T cells was confirmed by stimulating lines in the absence of APCs with plate-bound anti-CD3 mAb after two rounds of Ag-specific stimulation. Moreover, IL-12 enhanced IL-10 and IFN-gamma production in a myelin basic protein-reactive T cell clone, demonstrating that a differentiated T cell clone could be induced to secrete both cytokines, The addition of a neutralizing anti-IFN-gamma Ab to cultures with IL-12 and anti-IL-4 mAb during the generation of tetanus toxoid-reactive lines had no effect on the induction of IL-10 and IFN-gamma secretion, indicating that IL-12 and not IFN-gamma was responsible for the induction of this subset of T cells, Thus, in human T cells, IL-12 induces concomitant secretion of IL-10 and IFN-gamma. |
| Document Type: Article |
| Language: English |
Addresses:
1. BRIGHAM & WOMENS HOSP, DEPT NEUROL, CTR NEUROL DIS, LAB MOL IMMUNOL, BOSTON, MA 02115 USA
2. HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA |
| Publisher: AMER ASSOC IMMUNOLOGISTS, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Immunology |
| IDS Number: VG999 |
| ISSN: 0022-1767 |
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