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| A male-specific role for SOX9 in vertebrate sex determination |
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| Author(s): Kent J, Wheatley SC, Andrews JE, Sinclair AH, Koopman P |
| Source: DEVELOPMENT Volume: 122 Issue: 9 Pages: 2813-2822 Published: SEP 1996 |
| Times Cited: 370 References: 68 |
| Abstract: Mutation analyses of patients with campomelic dysplasia, a bone dysmorphology and XY sex reversal syndrome, indicate that the SRY-related gene SOX9 is involved in both skeletal development and sex determination. To clarify the role SOX9 plays in vertebrate sex determination, we have investigated its expression during gonad development in mouse and chicken embryos, In the mouse, high levels of Sox9 mRNA were found in male (XY) but not female (XX) genital ridges, and were localised to the sex cords of the developing testis, Purified fetal germ cells lacked Sox9 expression, indicating that Sox9 expression is specific to the Sertoli cell lineage, Sex specificity of SOX9 protein expression was confirmed using a polyclonal antiserum, The timing and cell-type specificity of Sox9 expression suggests that Sox9 may be directly regulated by SRY, Male-specific expression of cSOX9 mRNA during the sex determination period was also observed in chicken genital ridges. The conservation of sexually dimorphic expression in two vertebrate classes which have significant differences in their sex determination mechanisms, points to a fundamental role for SOX9 in testis determination in vertebrates. Sox9 expression was maintained in the mouse testis during fetal and adult life, but no expression was seen at any stage by in situ hybridisation in the developing ovary, Male-specific expression was also observed in the cells surrounding the Mullerian ducts and in the epididymis, and expression in both sexes was detected in the developing collecting ducts of the metanephric kidney, These results suggest that SOX9 may have a wider role in the development of the genitourinary system.
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| Document Type: Article |
| Language: English |
| Reprint Address: Kent, J (reprint author), UNIV QUEENSLAND, CTR MOL & CELLULAR BIOL, BRISBANE, QLD 4072 AUSTRALIA |
Addresses:
1. UNIV QUEENSLAND, DEPT ANAT SCI, BRISBANE, QLD 4072 AUSTRALIA
2. UNIV MELBOURNE, ROYAL CHILDRENS HOSP, DEPT PAEDIAT, MELBOURNE, VIC 3052 AUSTRALIA
3. UNIV MELBOURNE, ROYAL CHILDRENS HOSP, CTR HORMONE RES, MELBOURNE, VIC 3052 AUSTRALIA |
| Publisher: COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL |
| Subject Category: Developmental Biology |
| IDS Number: VJ075 |
| ISSN: 0950-1991 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |