| | |  | | | | Record from Web of Science® | | | | | | |
| Accessing genetic information with high-density DNA arrays |
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| Author(s): Chee M, Yang R, Hubbell E, Berno A, Huang XC, Stern D, Winkler J, Lockhart DJ, Morris MS, Fodor SPA |
| Source: SCIENCE Volume: 274 Issue: 5287 Pages: 610-614 Published: OCT 25 1996 |
| Times Cited: 1,010 References: 31 |
| Abstract: Rapid access to genetic information is central to the revolution taking place in molecular genetics. The simultaneous analysis of the entire human mitochondrial genome is described here, DNA arrays containing up to 135,000 probes complementary to the 16.6-kilobase human mitochondrial genome were generated by light-directed chemical synthesis, A two-color labeling scheme was developed that allows simultaneous comparison of a polymorphic target to a reference DNA or RNA. Complete hybridization patterns were revealed in a matter of minutes. Sequence polymorphisms were detected with single-base resolution and unprecedented efficiency, The methods described are generic and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability. |
| Document Type: Article |
| Language: English |
| Reprint Address: Chee, M (reprint author), AFFYMETRIX, 3380 CENT EXPRESSWAY, SANTA CLARA, CA 95051 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: VN919 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |