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| Use of a cDNA microarray to analyse gene expression patterns in human cancer |
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| Author(s): DeRisi J, Penland L, Brown PO, Bittner ML, Meltzer PS, Ray M, Chen YD, Su YA, Trent JM |
| Source: NATURE GENETICS Volume: 14 Issue: 4 Pages: 457-460 Published: DEC 1996 |
| Times Cited: 1,269 References: 22 |
| Abstract: The development and progression of cancer(1-3) and the experimental reversal of tumorigenicity(4,5) are accompanied by complex changes in patterns of gene expression. Microarrays of cDNA provide a powerful tool for studying these complex phenomena(6-8). The tumorigenic properties of a human melanoma cell line, UACC-903, can be suppressed by introduction of a normal human chromosome 6, resulting in a reduction of growth rate, restoration of contact inhibition, and suppression of both soft agar clonogenicity and tumorigenicity in nude mice(4,5,9). We used a high density microarray of 1,161 DNA elements to search for differences in gene expression associated with tumour suppression in this system. Fluorescent probes for hybridization were derived from two sources of cellular mRNA [UACC-903 and UACC-903(+6)] which were labelled with different fluors to provide a direct and internally controlled comparison of the mRNA levels corresponding to each arrayed gene. The fluorescence signals representing hybridization to each arrayed gene were analysed to determine the relative abundance in the two samples of mRNAs corresponding to each gene. Previously unrecognized alterations in the expression of specific genes provide leads for further investigation of the genetic basis of the tumorigenic phenotype of these cells. DNA microarrays, containing 1,161 total elements. |
| Document Type: Article |
| Language: English |
Addresses:
1. STANFORD UNIV, MED CTR, DEPT BIOCHEM, STANFORD, CA 94305 USA
2. STANFORD UNIV, MED CTR, HOWARD HUGHES MED INST, STANFORD, CA 94305 USA
3. NIH, NATL CTR HUMAN GENOME RES, CANC GENET LAB, BETHESDA, MD 20892 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: VV730 |
| ISSN: 1061-4036 |
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