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| A monoclonal blocking-ELISA for detection of orthopoxvirus antibodies in feline sera |
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| Author(s): Czerny CP, Wagner K, Gessler K, Mayr A, Kaaden OR |
| Source: VETERINARY MICROBIOLOGY Volume: 52 Issue: 3-4 Pages: 185-200 Published: OCT 1996 |
| Times Cited: 12 References: 46 |
| Abstract: A double sandwich blocking-ELISA using a genus-specific neutralizing monoclonal antibody (MAb) against the vaccinia virus 32 kD adsorption protein (D8L open reading frame; ORF) was developed to detect orthopoxvirus (OPV) antibodies in sera. A collection of 2173 feline serum samples was examined in an epidemiological study. The blocking-ELISA revealed 44 (2%) sera with positive titres of 1:2-1:256. ELISA results were confirmed by the plaque-reduction test. A close correlation between titres of both assays could be observed (r = 0.986). In general, the sensitivity of the blocking ELISA was two to four times higher. Neutralizing OPV-antibodies were found in nine sera with ELISA-titres > 1:4. Antibody specificity to OPV was also demonstrated by Western blotting analysis with selected feline sera. The epidemiographical distribution of the ELISA-positive sera and case histories of 37 seropositive cats available from the referring veterinarians are demonstrated. The blocking-ELISA enables a rapid serological diagnosis and can be used in veterinary and human medicine. It allows OPV-antibody screening in human and other animal species. |
| Document Type: Article |
| Language: English |
| Reprint Address: Czerny, CP (reprint author), UNIV MUNICH, INST MED MICROBIOL INFECT & EPIDEM DIS, VET STR 13, D-80539 MUNICH, GERMANY |
Addresses:
1. UNIV MUNICH, FAC VET, WHO, COLLABORAT CTR COLLECT & EVALUAT DATA COMPARAT VI, D-80539 MUNICH, GERMANY
2. NATL VET INST, HEIDELBERG, GERMANY |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Microbiology; Veterinary Sciences |
| IDS Number: VW556 |
| ISSN: 0378-1135 |
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