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| Morphological analysis of germ cell apoptosis during postnatal testis development in normal and hsp 70-2 knockout mice |
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| Author(s): Mori C, Nakamura N, Dix DJ, Fujioka M, Nakagawa S, Shiota K, Eddy EM |
| Source: DEVELOPMENTAL DYNAMICS Volume: 208 Issue: 1 Pages: 125-136 Published: JAN 1997 |
| Times Cited: 71 References: 55 |
| Abstract: The present study examined the occurrence of apoptotic cell death in the testis of wild-type mice from postnatal days 3 to 28 and in juvenile Hsp70-2 knockout mice. Adult Hsp70-2 knockout males are infertile and lack spermatids and spermatozoa (Dir et al. [1996a] Proc. Natl. Acad. Sci. U.S.A. 93:3264-3268). To identify the cell types undergoing apoptosis, we also examined the relationship between the occurrence of apoptotic cell death and the expression pattern of the Hsp70-2 gene product (heat-shock protein 70-2 [Hsp70-2]; marker for spermatocytes and spermatids), germ cell nuclear antigen 1 (GCNA1; marker for spermatogonia and spermatocytes), and vimentin (marker for Sertoli cells). This study shows that during postnatal development of the wildtype mouse testis (1) the percentage of apoptotic cell death detected by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method is higher in mice from days 8 to 22 than in younger or older mice, (2) the majority of apoptotic cells are spermatogonia and less frequently are spermatocytes, and (3) the degenerative cell death of spermatogonia and primary spermatocytes involves apoptosis with fragmentation of DNA. The analysis of apoptotic cell death in the testes of juvenile Hsp70-2 knockout mice showed an additional increased level of apoptosis at day 17, during the first wave of spermatogenesis, in pachytene spermatocytes. (C) 1997 Wiley-Liss, Inc. |
| Document Type: Article |
| Language: English |
| Reprint Address: Mori, C (reprint author), KYOTO UNIV, FAC MED, DEPT ANAT & DEV BIOL, SAKYO KU, KYOTO 60601, JAPAN |
Addresses:
1. KYOTO UNIV, FAC MED, CENT LAB ELECTRON MICROSCOPY, KYOTO, JAPAN
2. NIEHS, GAMETE BIOL SECT, REPROD & DEV TOXICOL LAB, NIH, RES TRIANGLE PK, NC 27709 USA
3. US EPA, REPROD TOXICOL DIV, NATL HLTH & ENVIRONM EFFECTS RES LAB, RES TRIANGLE PK, NC 27711 USA |
| Publisher: WILEY-LISS, DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 |
| Subject Category: Anatomy & Morphology; Developmental Biology |
| IDS Number: WA756 |
| ISSN: 1058-8388 |
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