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| HLA-DR polymorphism modulates the cytokine profile of Mycobacterium leprae HSP-reactive CD4(+) T cells |
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| Author(s): Mitra DK, Rajalingam R, Taneja V, Bhattacharyya BC, Mehra NK |
| Source: CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY Volume: 82 Issue: 1 Pages: 60-67 Published: JAN 1997 |
| Times Cited: 15 References: 24 |
| Abstract: In the present study, in vitro attempts have been made to define the cytokine profile of CD4(+) T cells from polar leprosy patients and healthy individuals against Mycobacterium leprae-derived heat shock proteins (HSPs), HSP65 and HSP18, and their trypsin-digested fragments, relating to HLA-DR polymorphism. While all tryptic fragments of optimal digestion and undigested HSPs could stimulate CD4(+) T cells from tuberculoid (TT) leprosy patients and healthy contacts (stimulation index, SI > 2.0), only two fragments, TDB65-2 (18 kDa) and TDB18-3 (3 kDa) triggered CD4(+) T cells of anergic lepromatous (LL) leprosy patients. Both of these HSPs and their tryptic fragments showed diverse HLA-DR restriction, with DR15 providing the strongest restriction, Cytokine analysis demonstrated that HSP65 and HSP18 induced Th1-like activity in the context of all the restricting HLA-DR alleles, except DR1 and DR7 which induced a Th2 type of response against HSP65 and HSP18, respectively. These Th2 inducer epitopes on HSP65 (DR1 restricted) and HSP18 (DR7 restricted) were absent from TDB65-2 and TDB18-3 which exclusively triggered Th1 cells in both TT and LL forms of leprosy in the context of multiple DR alleles, DR15 being the major antigen-presenting allele. These studies suggest that the major histocompatibility complex phenotype of the antigen-presenting cell can modulate Th1-like versus Th2-like activity against M. leprae pathogens in leprosy and healthy individuals. (C) 1997 Academic Press, Inc. |
| Document Type: Article |
| Language: English |
| Reprint Address: Mitra, DK (reprint author), ALL INDIA INST MED SCI, HISTOCOMPATIBIL & IMMUNOGENET DEPT, NEW DELHI 110029, INDIA |
Addresses:
1. INDIAN INST TECHNOL, DEPT CHEM ENGN, BIOTECHNOL UNIT, MIDNAPORE 721302, W BENGAL INDIA |
| Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 |
| Subject Category: Immunology; Pathology |
| IDS Number: WC932 |
| ISSN: 0090-1229 |
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