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| Genomic organization and expression of the human fatty aldehyde dehydrogenase gene (FALDH) |
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| Author(s): Rogers GR, Markova NG, DeLaurenzi V, Rizzo WB, Compton JG |
| Source: GENOMICS Volume: 39 Issue: 2 Pages: 127-135 Published: JAN 15 1997 |
| Times Cited: 31 References: 25 |
| Abstract: Mutations in the fatty aldehyde dehydrogenase (FALDH) gene cause Sjogren-Larsson syndrome (SLS)-a disease characterized by mental retardation, spasticity, and congenital ichthyosis. To facilitate mutation analysis in SLS and to study the pathogenesis of FALDH deficiency, we have determined the structural organization and characterized expression of the FALDH (proposed designation ALDH10) gene. The gene consists of 10 exons spanning about 30.5 kb. A TATA-less promoter is associated with the major transcription initiation site found to be 258 bp upstream of the ATG codon The GC-rich sequences surrounding the transcription initiation site encompassed regulatory elements that interacted with proteins in HeLa nuclear extracts and were able to promote transcription in vitro. FALDH is widely expressed as three transcripts of 2, 3.8, and 4.0 kb, which originate from multiple polyadenylation signals in the 3' UTR. An alternatively spliced mRNA was detected that contains an extra exon and encodes an enzyme that is likely to have altered membrane-binding properties. The FALDH gene lies only 50-85 kb hom ALDH3, an aldehyde dehydrogenase gene that has homologous sequence and intron/exon structure. (C) 1997 Academic Press |
| Document Type: Article |
| Language: English |
Addresses:
1. NIAMSD, NIH, GENET STUDIES SECT, SKIN BIOL LAB, BETHESDA, MD 20892 USA
2. UNIV ROMA TOR VERGATA, DEPT EXPT MED, BIOCHEM LAB, IST DERMOPAT IMMACOLATA, IRCCS, I-00167 ROME, ITALY
3. VIRGINIA COMMONWEALTH UNIV, MED COLL VIRGINIA, DEPT PEDIAT, RICHMOND, VA 23298 USA
4. VIRGINIA COMMONWEALTH UNIV, MED COLL VIRGINIA, DEPT HUMAN GENET, RICHMOND, VA 23298 USA |
| Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 |
| Subject Category: Biotechnology & Applied Microbiology; Genetics & Heredity |
| IDS Number: WE849 |
| ISSN: 0888-7543 |
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