| | |  | | | | Record from Web of Science® | | | | | | |
| Characterization of the yeast transcriptome |
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| Author(s): Velculescu VE, Zhang L, Zhou W, Vogelstein J, Basrai MA, Bassett DE, Hieter P, Vogelstein B, Kinzler KW |
| Source: CELL Volume: 88 Issue: 2 Pages: 243-251 Published: JAN 24 1997 |
| Times Cited: 647 References: 34 |
| Abstract: We have analyzed the set of genes expressed from the yeast genome, herein called the transcriptome, using serial analysis of gene expression. Analysis of 60,633 transcripts revealed 4,665 genes, with expression levels ranging from 0.3 to over 200 transcripts per cell. Of these genes, 1981 had known functions, while 2684 were previously uncharacterized. The integration of positional information with gene expression data allowed for the generation of chromosomal expression maps identifying physical regions of transcriptional activity and identified genes that had not been predicted by sequence information alone. These studies provide insight into global patterns of gene expression in yeast and demonstrate the feasibility of genome-wide expression studies in eukaryotes. |
| Document Type: Article |
| Language: English |
| Reprint Address: Velculescu, VE (reprint author), JOHNS HOPKINS UNIV, SCH MED, PROGRAM HUMAN & GENET & MOL BIOL, BALTIMORE, MD 21231 USA |
Addresses:
1. JOHNS HOPKINS UNIV, SCH MED, CTR ONCOL, BALTIMORE, MD 21231 USA
2. JOHNS HOPKINS UNIV, SCH MED, HOWARD HUGHES MED INST, BALTIMORE, MD 21231 USA
3. JOHNS HOPKINS UNIV, SCH MED, DEPT MOL BIOL & GENET, BALTIMORE, MD 21231 USA
4. NATL LIB MED, NATL CTR BIOTECHNOL INFORMAT, BETHESDA, MD 20894 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: WE970 |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |