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| Human chromosomal fragile site FRA16B is an amplified AT-rich minisatellite repeat |
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| Author(s): Yu S, Mangelsdorf M, Hewett D, Hobson L, Baker E, Eyre HJ, Lapsys N, LePaslier D, Doggett NA, Sutherland GR, Richards RI |
| Source: CELL Volume: 88 Issue: 3 Pages: 367-374 Published: FEB 7 1997 |
| Times Cited: 132 References: 38 |
| Abstract: Fragile sites are nonstaining gaps in chromosomes induced by specific tissue culture conditions. They vary both in population frequency and in the culture conditions required for induction. Folate-sensitive fragile sites are due to expansion of p(CCG)(n) trinucleotide repeats; however, the relationship between sequence composition and the chemistry of induction of fragile sites is unclear. To clarify this relationship, the distamycin A-sensitive fragile site FRA16B was isolated by positional cloning and found to be an expanded 33 bp AT-rich minisatellite repeat, p(ATATATTATATATTATATCTAATAATATAT(C)/(A)TA)(n) (consistent with DNA sequence binding preferences of chemicals that induce its cytogenetic expression). Therefore the mutation mechanism associated with trinucleotide repeats is also a property of minisatellite repeats (variable number tandem repeats). |
| Document Type: Article |
| Language: English |
| Reprint Address: Yu, S (reprint author), WOMENS & CHILDRENS HOSP, DEPT CYTOGENET & MOL GENET, CTR MED GENET, ADELAIDE, SA 5006 AUSTRALIA |
Addresses:
1. UNIV ADELAIDE, DEPT GENET, ADELAIDE, SA 5000 AUSTRALIA
2. LOS ALAMOS NATL LAB, DIV LIFE SCI, LOS ALAMOS, NM 87545 USA
3. FDN JEAN DAUSSET, CTR ETUD POLYMORPHISME HUMAIN, PARIS, FRANCE |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: WG479 |
| ISSN: 0092-8674 |
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