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| A general primer GP5+/GP6+-mediated PCR-enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings |
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| Author(s): Jacobs MV, Snijders PJF, vandenBrule AJC, Helmerhorst TJM, Meijer CJLM, Walboomers JMM |
| Source: JOURNAL OF CLINICAL MICROBIOLOGY Volume: 35 Issue: 3 Pages: 791-795 Published: MAR 1997 |
| Times Cited: 288 References: 30 |
| Abstract: Two cocktails of digoxigenin-labeled human papillomavirus (HPV) type-specific oligonucleotide probes and an enzyme immunoassay (EIA) were used as a basis to develop a group-specific detection method for 14 high-risk (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) and 6 low-risk (types 6, 11, 40, 42, 43, and 44) HPVs, following a general primer GP5(+)/bioGP6(+)-mediated PCR, The sensitivity of this high-risk/low-risk (HR/LR) HPV PCR-EIA ranged from 10 to 200 HPV copies, depending on the HPV type. Comparison of HR/LR HPV PCR-EIA with radioactive Southern blot hybridization using a general probe on the same PCR products derived from 417 cytomorphologically abnormal cervical scrapings resulted in an overall agreement of 96% between the two methods. Complete concordance between group-specific HR/LR HPV detection and individual typing results for both single and multiple infections indicate the strong specificity of this HR/LR HPV PCR-EIA. Multiple infections could be predicted by comparing PCR-EIA optical density values of the cocktail probes with one of the individual oligonucleotide probes. This novel HR/LR HPV PCR-EIA allows accurate and rapid identification of high-risk and low-risk HPV types in cervical scrapings and will facilitate HPV detection in HPV mass-screening programs. |
| Document Type: Article |
| Language: English |
Addresses:
1. FREE UNIV AMSTERDAM HOSP, DEPT PATHOL, SECT MOL PATHOL, NL-1081 HV AMSTERDAM, NETHERLANDS
2. FREE UNIV AMSTERDAM HOSP, DEPT OBSTET & GYNECOL, NL-1081 HV AMSTERDAM, NETHERLANDS |
| Publisher: AMER SOC MICROBIOLOGY, 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 |
| Subject Category: Microbiology |
| IDS Number: WJ848 |
| ISSN: 0095-1137 |
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